Construction of a BAC library and mapping BAC clones to the linkage map of Barramundi, Lates calcarifer

A microsatellite linkage map of Barramundi, Lates calcarifer.

Barramundi (Lates calcarifer) is an important farmed marine food fish species. Its compact genome (approximately 700 Mb) is among the smallest genomes of food fish species. We established a first-generation genetic linkage map of Barramundi with a mapping panel containing three parents (two males and one female) and 93 progeny. A total of 240 microsatellite markers were mapped into 24 linkage g...

A First Generation BAC-Based Physical Map of the Asian Seabass (Lates calcarifer)

BACKGROUND The Asian seabass (Lates calcarifer) is an important marine foodfish species in Southeast Asia and Australia. Genetic improvement of this species has been achieved to some extent through selective breeding programs since 1990s. Several genomic tools such as DNA markers, a linkage map, cDNA and BAC libraries have been developed to assist selective breeding. A physical map is still lac...

BAC library construction.

Screening of Sunflower BAC Library for the Identification of Specific BAC Clones

To facilitate molecular cytogenetic research and the integration of cytogenetic and genetic linkage groups of sunflower, one BAC and one BIBAC library from HA 89 with BamHI and HindIII, respectively, were developed. The average insert sizes were estimated to be 140 kb and 137 kb in the BamHI and HindIII libraries, respectively. The combined libraries consist of 192,000 clones and represent appr...

Defining essential fatty acid deficiency in juvenile barramundi Lates calcarifer

Barramundi (Lates calcarifer), a catadromous teleost of significant and growing commercial importance, are reported to have limited fatty acid bioconversion capability, typical of marine species, and therefore require preformed long-chain polyunsaturated fatty acids (LC-PUFA) as dietary essential fatty acid (EFA). In this study, the response of juvenile barramundi (47.0 g/fish initial weight) f...